Optomotor project nearing completion
on Monday, September 4th, 2023 1:59 | by Björn Brembs
The results of comparing optomotor responses after self-learning remain solid. There still is a small asymmetry between the left/right groups, but nothing dramatic:
Category: operant self-learning, Optomotor response | No Comments
Cloning via DNA Assembly
on Friday, September 1st, 2023 7:26 | by Isabel Stark
DNA Assembly in a 1:2 ratio of vector to insert with gRNAs of rsh and rut (Q5 and template concentration: 640 pg/µl) and 100ng of pCFD6 BbsI AP (using QuickCIP). Heat-shock (hs) transformation into E. coli (DH5α competent) with 10 µl Assembly Reaction and 100 µl cells.
For Crtl, pCFD6 BbsI AP was wrongly used.
DNA Assembly in a 1:2 ratio of vector to insert with the gRNAs of rsh and rut (Q5 and template concentration: 640 pg/µl) and 100ng of pCFD6 BbsI AP (using FastAP and 2 extraction steps). Heat-shock (hs) transformation into E. coli (DH5α competent) with 10 µl Assembly Reaction and 100 µl cells.
For Crtl, pCFD6 BbsI AP was used in reaction.
Category: genetics, Memory, Operant learning, operant self-learning, Radish | No Comments
Bachelor Blog / #4 is there something?
on Monday, August 7th, 2023 2:13 | by Ellie
The offspring of my first experimental fly cohort finally hatched! Below you find a few first pre-tests I ran last week :)
First, here are the results of a quick test to see if the offspring shows a preference for the parentally trained side after the first training period:
After that I played around with the laser a little bit to find the learning threshhold. I set the laser on 2,6V but the results I got look a bit weird:
-> untrained wtb flies
-> offspring of trained wtb flies
I´m optimistic however there is still a lot of work to do…
Success: rsh Stock has rsh1 Mutation
on Monday, August 7th, 2023 11:11 | by Isabel Stark
Via gDNA analysis and PCR was the specific area of the rsh gene extracted and amplified where the nucleotide substitution: C to T (Folkers et al., 2006) should be for the rsh1 mutation. The amplicon was Sanger sequenced which proved the nucleotide substitution.
Category: genetics, Memory, Operant learning, operant self-learning, Radish, Uncategorized | No Comments
Little by little
on Monday, July 24th, 2023 1:59 | by Björn Brembs
Despite very warm weather, some flies did fly, even though the learning performance of the control flies was really poor. At least for now, it looks like all stocks are learning and that rut and rsh flies learn at least equally well as the Berlin flies. I’ve also managed to fix the positive preference problem:
Category: operant self-learning, Radish | No Comments
Creating gRNAs via PCR
on Friday, July 7th, 2023 6:38 | by Isabel Stark
Category: genetics, Memory, Operant learning, operant self-learning, Radish, Uncategorized | No Comments
Kicked out Canton S
on Thursday, June 29th, 2023 5:25 | by Björn Brembs
Since the Canton S strain I used wasn’t a perfect genetic background strain anyway and didn’t fly properly, I kicked it out and replaced it with the wild type Berlin data I had collected just prior to the rut and rsh mutants. Now I can collect data for two projects in one go: I check the rut/rsh learning mutants if they still behave the way they should and with the wtb control strain and continue collecting data to evaluate their optomotor responses after training. So now the learning scores for the three strains look like this:
It still doesn’t look like rut is better than wtb, so I’m still skeptical that the strain is really what it should be. The rsh data also don’t look very promising, but I still need to get more flies with a negative preference before the training.
Category: operant self-learning, Optomotor response | No Comments
Suspicion and progress!
on Monday, June 26th, 2023 2:03 | by Björn Brembs
Suspicion: rut may not be rut
Carsten Duch is identifying the muscles involved in operant self-learning:
Category: operant self-learning | No Comments
It’s a start
on Friday, June 16th, 2023 4:42 | by Björn Brembs
The first few rutabaga and radish mutants have been measured and so far it looks good: standard duration yaw torque learning – the situation all three groups should learn.
Category: Operant learning, operant self-learning | No Comments