T-Maze experiments : screen results as on 02-07-2018

on Monday, July 2nd, 2018 2:09 | by

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

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Modelling the valence of dopaminergic clusters from the Y-mazes

on Monday, June 25th, 2018 1:52 | by

Dopaminergic clusters are differently targeted by the different Gal4s. Some of the express faintly, others stronger. Here I try to see if the dose-response curve (or expression-PI curve) seems to be linear or not. Here I put two examples from the 17 clusters, where the first two seem to have nonlinear curves, with and optimal expression level, and the last two seem to have a linear response curve.

This will be important for the modelling in order to decide to make a linear/nonlinear model. Down below I show the results from a linear model and it´s statistics. From Aso et al. 2012, one could see that activating the lines with TrpA1 shows a linear response curve. But in this case it does not necessarily seem to be the case. Therefore, light intensities might have an effect, as well as the expression level, and conclusion needs to be taken carefully.

 

In addition it is difficult to calculate this for all the clusters with just one single light intensity test, because not all clusters are expressed in several Gal4s to different level, so that we can estimate from there. So for the interesting lines we might need to make several experiments at different intensities, and see the dose response curve.

 

The G4s I have used for the modelling are the ones shown here.

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Making new ratios for Y-maze

on Monday, June 18th, 2018 1:55 | by

This is just to show that I am trying to find  a new ratio so that all graphs have from -1 to +1 ranges. That is why now the difference in occupancy time is divided by the total time. The same with the speed. Because speed differences are so subtle, the Y axis scale has to be lower

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Tmaze experiments initial results

on Monday, June 4th, 2018 2:16 | by

the first graph shows Bar plot of Mean and standard deviation of PIs for Genetic control (n=9) and Positive control (n=5).
The second graph shows weighted mean and weighted standard deviation of the same.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

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Summary of results of master thesis

on Monday, December 11th, 2017 12:35 | by

Self-learning of Rover/sitter from flight simulator

Long-term memroy from self-learning

 

Self-learning training/tests from transgeneic flies

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Chrimson/Gr28bd lines tests in yellow light (580nm)

on Monday, December 4th, 2017 2:17 | by

 

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new TrpA/chrimson self-learning tests

on Monday, November 27th, 2017 12:05 | by

NorpA; Chrimson/Gr28 (N=12, ATR)

NorpA; Chrimson/Gr28 (N012, No ATR)

NorpA;Chrimson/+ (N=11, ATR)

 

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Normal vision on platform test

on Monday, October 2nd, 2017 11:08 | by

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Self learning test and long-term memory test of TrpA1 from optogenetic training

on Monday, September 4th, 2017 2:20 | by

TrpA1 self learning test, N= 47

TrpA1 long term memory, N= 39

Wtb long term memory (previous on fly simulator), N= 16

 

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self-learning update

on Friday, July 21st, 2017 4:11 | by

N= 31

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