longterm memory test

on Monday, June 19th, 2017 1:55 | by

N= 30, p-value = 0.66

 

Rescue from forS in visual memory defects:

adult-limit for expression in fan-shape body (c205-Gal80ts)

increase for expression in ellipsoid body (c819-Gal80ts)

 

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R,S,WTB updates, and methods to inhibate PKG

on Monday, June 12th, 2017 10:31 | by

Rover,N= 36

Sitter, N= 28

WTB, N= 13

PKG affects K channels

Two ways to inhibitatePKG:

1

UAS-Gal4, RNAi

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/+

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/ UAS-for RNAi

2

PKG inhibitors:

  1. KT 5823(10mM in DMSO)
  2. (irrversible)

 

 

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Updates on R,S,WTB tests

on Friday, May 26th, 2017 1:20 | by

Rover, N=3

sitter, N=2

wtb, N=4

longterm, N=13 (old WTB excluded)

R,S self learning traces, and longterm memory trace

WTB self learning traces, longterm memory trace

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update on rover&sitter test and longterm memory test

on Friday, May 12th, 2017 3:41 | by

R=30

S=20

longterm memory N= 22

control N= 6

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update on self learning&longterm memory

on Monday, April 3rd, 2017 1:44 | by

longterm test:

1 from sitter,  8 from wtb, 3 from rover (total N = 12)

mean(test): 0.248          SD(test): 0.7

Longterm test Control

1 from sitter, 2 from rover (N=3)

mean(test):-0.386           SD(test):0.79

Two Sample:

Based on 50%(20%) of statistical power, the minimum sample size is 7 (17)for both group.

One Sample (assume control group has the average of PI is 0):

Based on 50% (20%) of statistical power, the minimum sample size is 22 (49)for both group.

https://www.dssresearch.com/KnowledgeCenter/toolkitcalculators/samplesizecalculators.aspx

 

Self learning test:

sitter N=5

wtb N= 4

 

 

 

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PKG rover update

on Monday, March 6th, 2017 10:17 | by

There is the result of 20 measurements on PKG rover.

pretest:-0.45

train1:0.56

train2:0.71

test1:0.47

train3:0.83

train4:0.87

test2:0.39

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Laser power/angle adjustment

on Monday, February 20th, 2017 10:15 | by

After camera and punish laser adjusted, flies showed a high avoidence in the trail. Especially, when the fly was hit by punish laser in the first time of each training block. Unfortunately, there was a huge drift in the last trace after adjustment, but it shows a good avoidence in each training block.

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PKG rover from last week

on Monday, February 13th, 2017 9:37 | by

Generally, PKG drosophila were flying much better when they hatched from normal temperature. There is the result from 10 PKG drosophila.

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Update on PKG sitter/rover PI

on Monday, February 6th, 2017 9:41 | by

There is a short update on summerized previous PI. Still, PKG transgenic drosophila is hardly testable, and the results seems not so promising. I’m going to focus on testing WTB this week, to compare the difference of PI between WTB and PKG

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First glance at PKG effect on self-learning and long term memory

on Monday, January 23rd, 2017 2:51 | by

There are two strains of drosophila prepared: PKG-rover(R), PKG-sitter(S). Each strain is supposed to test PI in 3 phases: no training, training, long term+no training. The following data show PKG-sitter flying trace and PI. Since there weren’t many flies in the first week, and they pause flying very often in the experiment, so there is only one single fly was tested successfully. Since the torque meter drifted on the next day, the long term result is quite debatable.

 

 

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