Update on DA & photopreference

on Monday, April 24th, 2017 1:55 | by

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

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Photopreference shift and DA (new and old GAL4s)

on Monday, April 3rd, 2017 1:56 | by

Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.

 

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Behavioral Test on DA-GAL4s

on Monday, March 27th, 2017 2:06 | by

I am currently testing different dopaminergic GAL4s in order to validate previous results from my initial screening. Here I can find the results for one of those GAL4s

 

 

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TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17 | by

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

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DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01 | by

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5
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TH-GAL4 subgroups (Anatomy)

on Monday, January 30th, 2017 11:42 | by

I have been doing crosses in order to establish different lines that will allow me to study the neurons behind the photopreference shift. Here I show the first dissections I did to test some of these lines.

Dopaminergic clusters (Left: posterior, Right: anterior)

My dissections

TH-C’ from the original publication (Top: anterior, Bottom: posterior)

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Short update on DA in photopreference

on Monday, January 23rd, 2017 2:16 | by

A few months ago I finished my first screen for DA-neuronal populations relevant in the photopreference shift. I tried a small collection of TH-Gal4s with interesting results that you could see here. To validate those results, I am currently searching for other Gal4s labeling the same groups of neurons. Here you can see the outcome of the first experiments for one of those lines.

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OA in photopreference (other GAL4s)

on Monday, January 16th, 2017 2:50 | by

Trying to define subsets of neurons related to the photopreference shift, I started to test the different OA and DA drivers available. Here you can see two OA-GAL4s used in Burke et al 2012:

My results:

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TbH-LexA: OA in photopreference (update)

on Monday, September 12th, 2016 2:06 | by

The following plot shows the photopreference of two LexA lines presumably labeling TbH neurons. Only wing-clipped flies are shown.

tbh-lexa-shits

 

26-09-2016, new experiments added.

tbh-lexa

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TbH QF system

on | by

In order to study the role of OA and DA in photopreference I am constantly looking for new drivers that label subpopulations of these groups. I recently found a TbH driver from the QF system, and wanted to know how representative of the TDC2-G4 neurons was. I have established a QUAS-mCherry;TbH-QFs line (Magenta) and crossed it with TDC2-G4;UAS-GFP (Green). In both cases, what is shown is the endogenous expression.

tdc2-gfptbhqfs-cherrytbhqftdc2-merge-fixation-protocoltdc2-gfp-vnctbhqfs-cherry-vnctbhqftdc2-vnc-merge-fixation-protocol

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