## Finding the interesting lines

on Friday, July 20th, 2018 3:46 | by Christian Rohrsen

This is the correlation from the T-maze experiments from Gaia and Naman. Neither ranked nor regular correlation show any significant effect. This means that these effects seem to be random, at least for most of them, is this an overfitting result?

I would say blue 1 is a line that was negative for all the tests I have so far seen. So this might be an interesting line. What to do next?

I would unblind the blue1, which is TH-D’. It was shown to be required for classical conditionning in shock and temperature learning (Galili et al. 2014). Another interesting observation is that th-g4+th-g80 seems to have like zero PI scores in all of the experiments (Naman and Gaia in the Tmaze, Joystick and Y-mazes). So could it be that all of these neurons have indeed a meaning, but is depending every time in the context?? Maybe Vanessa Ruta´s work might be interesting for that.

## T-Maze experiments : screen results as on 16-07-2018

on Monday, July 16th, 2018 1:32 | by Gaia Bianchini

Results of the T-maze screen analysis, both individual and combined.

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

## The T-Maze experiments : Middle analysis and Correlation plot

on Saturday, July 14th, 2018 12:06 | by Naman Agrawal

The mean ratio of the flies that stay in the middle during the experiments.

Correlation plot between the mean ratio of the flies that stay in the middle versus the Weighted PIs

Slope = 0.0053

Intercept = 0.240

R square value = -0.03834

contrary to the expectations, there seems to be no correlation .

Category: lab.brembs.net, neuronal activation, operant self-learning, Optogenetics, R code | No Comments

## T-Maze experiments : screen results as on 02-07-2018

on Monday, July 2nd, 2018 2:09 | by Gaia Bianchini

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:

Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

## Tmaze Exploratory analysis : Middle Flies

on | by Naman Agrawal

plotting the mean ratio of flies which stay in the middle during experiment.

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:

Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Category: genetics, neuronal activation, open science, Optogenetics | No Comments

## More on valence inference

on | by Christian Rohrsen

This is the linear model with its statistics

This is the linear model adding interactions. It is perfectly possible to have interactions between neurons, kind of what occurs with olfactory processing where ORNs activated alone or in different combinations have completely different meanings

I uploaded in slack the bayesian linear model with interactions. For any reason, it does not let me upload it now to the website

I am trying one of the ways of nonlinear models: GAM (Generalized additive models). Here one fit splines to the effects to certain degrees of freedom.

This is the kind of bar graphs I thought I could use for all the plots.

Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments

## Modelling the valence of dopaminergic clusters from the Y-mazes

on Monday, June 25th, 2018 1:52 | by Christian Rohrsen

Dopaminergic clusters are differently targeted by the different Gal4s. Some of the express faintly, others stronger. Here I try to see if the dose-response curve (or expression-PI curve) seems to be linear or not. Here I put two examples from the 17 clusters, where the first two seem to have nonlinear curves, with and optimal expression level, and the last two seem to have a linear response curve.

This will be important for the modelling in order to decide to make a linear/nonlinear model. Down below I show the results from a linear model and it´s statistics. From Aso et al. 2012, one could see that activating the lines with TrpA1 shows a linear response curve. But in this case it does not necessarily seem to be the case. Therefore, light intensities might have an effect, as well as the expression level, and conclusion needs to be taken carefully.

In addition it is difficult to calculate this for all the clusters with just one single light intensity test, because not all clusters are expressed in several Gal4s to different level, so that we can estimate from there. So for the interesting lines we might need to make several experiments at different intensities, and see the dose response curve.

The G4s I have used for the modelling are the ones shown here.

## T-Maze experiments : screen results as on 25-06-2018

on | by Naman Agrawal

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:

Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Category: genetics, lab.brembs.net, neuronal activation, open science, Operant learning, Optogenetics | No Comments

## Making new ratios for Y-maze

on Monday, June 18th, 2018 1:55 | by Christian Rohrsen

This is just to show that I am trying to find a new ratio so that all graphs have from -1 to +1 ranges. That is why now the difference in occupancy time is divided by the total time. The same with the speed. Because speed differences are so subtle, the Y axis scale has to be lower

## Initial screen results

on | by Gaia Bianchini

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

Category: crosses, genetics, lab.brembs.net, neuronal activation, open science, Operant learning, Optogenetics | No Comments