Results for amon-RNAi and MB143B

on Monday, February 26th, 2024 12:17 | by

The first figure shows the results for the preference test (salt/pure) conducted with amon-RNAi only under red light.

For the next experiment I conducted the same preference test but with the split-gal4 line MB143B under red and blue light.

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Results for MB+DANs and MB054B

on Monday, September 25th, 2023 11:07 | by

The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:

Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4

Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4

Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4

The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following:

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Verification TH-C-AD;TH-D-DBD

on Monday, September 11th, 2023 9:52 | by

Confocal images with anti-GFP and anti-Brp staining of TH-C-AD;TH-D-DBD > mCD::GFP fly brains: A: 4 PPM 2 DANs per hemisphere marked with blue circles, three clusters of Kenyon cell bodies per hemisphere marked with white arrows. B: Five visible cell bodies of unidentified neurons marked with a blue circle in the left hemisphere. The neurons project into the lobula plate and the medulla. Strong fluorescence of cell bodies of the Kenyon cells projecting into the Mushroom bodies is visible. C: Unidentified neurons projecting into structures outside of the optic lobes especially the ventral lateral protocerebrum.
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Optogenetic stimulation of DANs

on Saturday, September 2nd, 2023 11:09 | by

With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.

The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:

As seen in the figures, there was no effect under blue light.

For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:

With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.

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Preferences with higher salt concentration

on Monday, August 7th, 2023 12:46 | by

The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.

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Pure vs Sugar Preference results

on Monday, July 24th, 2023 9:24 | by

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Premature habit formation: neural circuitry

on Monday, May 8th, 2023 10:18 | by

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Inhibited subset of KCs in switch mode/yaw torque paradigm

on Friday, May 5th, 2023 3:52 | by

MB417B -> α’/β’ap, α’/β’m, α/βs, α/βp
MB463B -> α’/β’ap
MB371B -> α/βp

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NWG Poster on MBON-02 regulating habit formation

on Thursday, March 23rd, 2023 12:18 | by

If you missed it at the Göttingen NWG meeting, you can download our poster from here:

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Lines Verification

on Monday, September 12th, 2022 9:55 | by

MB371B

MB417B

MB463B

MB063B

MB194B

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