17d

on Monday, September 10th, 2018 1:03 | by

Been working on 17d for the past month.

The experiments in the flight simulator. Self-learning performance indices in a two-minute test with the heat switched off after 4 and 8 minutes of training, indicated impairment of 17d-TNT flies.

The flies also showed clear impairments in their flight performance. To quantify this I assessed both possible alterations in their motor coordination (using climbing assay) as well as flight performance. The climbing assay relies on walking rather than flying. Both experiments show reduced ability of motor coordination and flight performance.

 

 

To confirm the specificity of the 17d-Gal4 fly I used the trans-tango flies.

Neuron, 96 (2017) 783-799. doi:10.1016/j.neuron.2017.10.011

 

The trans-tango is notorious for having a low expression in adult flies, which was also observed by me. The image is taking without any GFP-antibody.

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Fussl shows numerical difference in operant self learning

on Tuesday, August 7th, 2018 2:49 | by

Fussl was crossed with either Stinger (ctrl) or a UAS-TNT line to block the synaptric transmission of the Fussl positive neurons. A third construct was used but did not yield any data due to difficulties with their flight performance. The Fussl-Stinger along with Fussl-TNT flies do also show difficulties in flying. These differences will be assessed.

The experiment was done as a pilot experiment before doing a larger scale.

The data is a bit inconsistent but shows a positive and reassuring numerical difference. The control is a bit lower than expected, compared to WTB flies (showing usually a PI 0f 0.6). The flies have a slightly different background than wtb flies and have pale orange eyes (still no apparent impairments in vision). Further experiments will be conducted before proceeding with a larger sample size of the flies.

 

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Role of dopaminergic neurons in operant behaviour

on Friday, July 27th, 2018 3:54 | by

Positive Control: Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

Red lines: completed

mb025b: not selected against tubby

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T-Maze experiments : screen results as on 16-07-2018

on Monday, July 16th, 2018 1:32 | by

Results of the T-maze screen analysis, both individual and combined.

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

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Crosses for isogenize FoxP-IsoB-Gal4 line

on Tuesday, June 19th, 2018 12:13 | by

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Category: crosses, Foxp | No Comments

Initial screen results

on Monday, June 18th, 2018 1:15 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

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CD8-GFP x FoxP-IsoB_Gal4

on Friday, June 8th, 2018 11:47 | by

Not confocal immages (going to the confocal this week)

The construct I am analyzing is the FoxP-IsoB_Gal4, so with the Gal4 inserted in the 8th exon

Larvae

Adult VNC

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Category: crosses, Foxp | No Comments

quality test before strokelitude experiments

on Monday, January 29th, 2018 12:40 | by

Some traces from this week just so that you have an idea how do they look like. To me they are not the optimal traces I expected. But one can see some signal there. I will start the screen hoping to get enough good traces without too much work.

what do you think is the best quality control for accepting a trace for the analysis or not. I was thinking the 3D mapping gives a good hint but without quantification.

In addition I was writting to Andi Straw to solve the issue of running two cameras in the same computers, but now answer

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Usage of TrpA with infrared lamp

on Wednesday, February 13th, 2013 11:40 | by

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