Test for hsGal4

on Monday, September 14th, 2020 1:40 | by

Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.

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General behavior of the MBON-2

on Monday, August 31st, 2020 12:54 | by

I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

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Progress Week 29

on Monday, July 20th, 2020 1:38 | by

-Introduced Sayani to the wonderful world of Drosophila

-Been doing some DTS coding

-Preparing flies to do optomotor response for Mathias Raß

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Progress week 26-28

on Monday, July 13th, 2020 1:39 | by

Updates in DTS code

Refreshing dissection skills

Tdc2-Gal4 expression in adult brain
Actin-GFP expression in adult brain

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Statistical evaluation of OR

on Monday, May 25th, 2020 12:23 | by

Recently I measured the optomotor response in T4/T5 flies. As expected, they did not respond to the optomotor stimulus as seen in the left chart below. However, statistical evaluation struggles to quantify this difference. It might be that this is because of the low sample size, or that we are using the wrong statistical analysis?

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Results from rescreen

on Monday, May 4th, 2020 1:49 | by

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Some issues with UAS-TNT control

on Monday, April 20th, 2020 1:57 | by

I experienced some issues with the first batch of the UAS-TNT control flies, theyh ad a very low learning curve. The second batched looked fine but the still have a slightly lower learning PI during Test1 than I want.

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Motion blindness in T4/T5 using Optomotor Response

on Tuesday, March 31st, 2020 1:48 | by

T4/T5 driver line was crossed TNT line to test for motion blindness using the optomoter response assay. While the controls respond perfectly fine to the rotating striped arena the T4/T5 flies are seemingly unaware of it. Sample size for T4/T5 flies is still a bit low. However, as this is only a trial experiment for the drosophila course I consider it successful.
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Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46 | by

  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis
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T-maze screen with yellow light

on Monday, February 3rd, 2020 12:57 | by

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