on Monday, April 8th, 2019 1:14 | by Miroslav Stojic
Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)
Trace (Downsampled 5) Graphs of Recordings
ElavGal4 x UAS-Ryr 28919:
ElavGal4 x UAS-Ryr 65885:
on Monday, April 1st, 2019 2:54 | by Miroslav Stojic
on Wednesday, March 20th, 2019 1:45 | by Anders Eriksson
A flight performance test to see how the flies are behaving while flying. They have a slightly lower flight duration compared to the genetic controls but nothing that should impair them in the experiment.
The learning experiments are ongoing. I am doing n=15 for each line and each experiment, both Composite Control and also a Isolated Operant experiment. The Composite control experiment is done but I only got 13 flies for Isolated Operant. I do not want to preanalyze the data and will therefor post it when all the flies are done.
on Monday, January 14th, 2019 2:54 | by Anders Eriksson
on Monday, October 29th, 2018 2:38 | by Anders Eriksson
on Monday, October 8th, 2018 2:32 | by Anders Eriksson
on Monday, October 1st, 2018 2:58 | by Anders Eriksson
on Monday, September 10th, 2018 1:03 | by Anders Eriksson
The experiments in the flight simulator. Self-learning performance indices in a two-minute test with the heat switched off after 4 and 8 minutes of training, indicated impairment of 17d-TNT flies.
The flies also showed clear impairments in their flight performance. To quantify this I assessed both possible alterations in their motor coordination (using climbing assay) as well as flight performance. The climbing assay relies on walking rather than flying. Both experiments show reduced ability of motor coordination and flight performance.
To confirm the specificity of the 17d-Gal4 fly I used the trans-tango flies.
The trans-tango is notorious for having a low expression in adult flies, which was also observed by me. The image is taking without any GFP-antibody.