Fussl shows numerical difference in operant self learning

on Tuesday, August 7th, 2018 2:49 | by

Fussl was crossed with either Stinger (ctrl) or a UAS-TNT line to block the synaptric transmission of the Fussl positive neurons. A third construct was used but did not yield any data due to difficulties with their flight performance. The Fussl-Stinger along with Fussl-TNT flies do also show difficulties in flying. These differences will be assessed.

The experiment was done as a pilot experiment before doing a larger scale.

The data is a bit inconsistent but shows a positive and reassuring numerical difference. The control is a bit lower than expected, compared to WTB flies (showing usually a PI 0f 0.6). The flies have a slightly different background than wtb flies and have pale orange eyes (still no apparent impairments in vision). Further experiments will be conducted before proceeding with a larger sample size of the flies.

 

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Role of dopaminergic neurons in operant behaviour

on Friday, July 27th, 2018 3:54 | by

Positive Control: Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

Red lines: completed

mb025b: not selected against tubby

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T-Maze experiments : screen results as on 16-07-2018

on Monday, July 16th, 2018 1:32 | by

Results of the T-maze screen analysis, both individual and combined.

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

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Tmaze Exploratory analysis : Middle Flies

on Monday, July 2nd, 2018 2:08 | by

plotting the mean ratio of flies which stay in the middle during experiment.

 

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

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T-Maze experiments : screen results as on 25-06-2018

on Monday, June 25th, 2018 1:13 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

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Crosses for isogenize FoxP-IsoB-Gal4 line

on Tuesday, June 19th, 2018 12:13 | by

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Category: crosses, Foxp | No Comments

Initial screen results

on Monday, June 18th, 2018 1:15 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

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Tmaze experiments : Test for Blindness and Comparison with positive control

on Monday, June 11th, 2018 12:45 | by

Comparison between White 1 ( Control (NorpA- UAS Chrimson)) and Yellow 1 (Positive Control ( Gr28bd-G4, TrpA1-G4).

To test whether the flies are really blind, and there is no problem with the NorpA part of the construct, we compare with NorpA,UAS GTACR1 ; NorpA,UAS GTACR2 ; and another stock of NorpA-UAS-Chrimson.

 

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

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CD8-GFP x FoxP-IsoB_Gal4

on Friday, June 8th, 2018 11:47 | by

Not confocal immages (going to the confocal this week)

The construct I am analyzing is the FoxP-IsoB_Gal4, so with the Gal4 inserted in the 8th exon

Larvae

Adult VNC

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Category: crosses, Foxp | No Comments

quality test before strokelitude experiments

on Monday, January 29th, 2018 12:40 | by

Some traces from this week just so that you have an idea how do they look like. To me they are not the optimal traces I expected. But one can see some signal there. I will start the screen hoping to get enough good traces without too much work.

what do you think is the best quality control for accepting a trace for the analysis or not. I was thinking the 3D mapping gives a good hint but without quantification.

In addition I was writting to Andi Straw to solve the issue of running two cameras in the same computers, but now answer

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