WB using the guinea pig antibody

on Friday, April 5th, 2019 2:05 | by

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Continuation of the LexA contruct cloning

on Tuesday, April 2nd, 2019 2:14 | by

Digestion of the construct which has Gal4. Gal4 must be removed and exchanged with LexA
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Cas9 controls show a bit of genetic background

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FoxP adult function

on Wednesday, March 27th, 2019 11:38 | by

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FoxPB-GABA larvae

on Friday, March 22nd, 2019 2:50 | by

VNC
central brain
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Buridan: UAS_gFoxP x D42_Gal4

on Monday, March 18th, 2019 2:56 | by

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FoxP-Gal4 construct

on Friday, March 15th, 2019 12:48 | by

FINALLYYYYYYYYYYYYYYYYYYYYYYYYYY!!!!!!!!!!!!!!
Test digestion shows the presence of the insert. the positive clones has been sent to sequencing
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GABA antibody

on Sunday, March 3rd, 2019 12:26 | by

No colocalization between FoxPB and GABA. FoxPB neurons are not GABAergic
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FoxPB Homo and Hetero

on Wednesday, February 27th, 2019 10:36 | by

I did it a third time just to be sure, in the meantime I am crossing with stinger in order to be able to count cells

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FoxPB + TH

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8 FoxPB cells are TH positive, and those 8 cells are conserved in all of the brains I checked
despite the presence of FoxPB-TH cells in the adult, I could not find any in the larvae
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