Hybridoma (Fusion) 1st screen

on Wednesday, October 24th, 2018 5:06 | by

This picture shows all of the positive clones in the ELISA from the 1st screen

While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA

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FoxPB adult cell count

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I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis

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FoxP-KO or WTB?

on Wednesday, October 17th, 2018 4:04 | by

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

 

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

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Protocerebral bridge in FoxP-KO flies

on Sunday, October 7th, 2018 6:06 | by

The more brains I see, the more I am convinced that the problem is not about my dissection

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MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)

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this result is still to be verified by deleting the red fluorescent eyes to the flies

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FoxP climbing assay

on Monday, October 1st, 2018 2:39 | by

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Counting of FoxP-isoB-Gal4 positive cells

on Wednesday, September 26th, 2018 10:19 | by

Counting of FoxP-isoB-Gal4 positive cells in adult and larvae brains (no VNC). Five brains counted for each age

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Tries with IMARIS

on Friday, September 21st, 2018 2:07 | by

Me and Anders spent some time at the imaris trying to get confidence with the software. We managed to do volume rendering and we counted the FoxP-isoB positive cells

  

the count resulted really similar to the one I´ve done by eyes:

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Protocerebral Bridge in FoxP-Gal4 and in FoxP-KO

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Protocerebral bridge in FoxP mutated flies

on Friday, September 14th, 2018 1:22 | by

The first 3 images show the protocerebral bridge in FoxP mutated flies. In the second line the first picture shows the protocerebral bridge in a FoxP isoB-Gal4 fly. The last image is taken and modifies from the Virtual Fly Brain

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