Test of 7 days old flies after GS
on Monday, February 15th, 2021 9:35 | by Andreas Ehweiner
Category: flight, Foxp, Memory, Operant learning, operant self-learning, Uncategorized | No Comments
Local FoxP knok out GMR11F02-GAL4
on | by Andreas Ehweiner
Category: flight, Foxp, Memory, Operant learning, operant self-learning, Uncategorized | No Comments
Preliminary test of 14 days old flies after GS
on Monday, January 25th, 2021 1:51 | by Andreas Ehweiner
With RU468 (2d), GSnsyb
No RU, GSnsyb
Category: flight, Foxp, Memory, Operant learning, operant self-learning | No Comments
GeneSwitch experiment
on Monday, January 18th, 2021 12:20 | by Sarah-Lynn Stratil
dFoxP knockout in embryonic stage
Category: Foxp | No Comments
FoxP in the fan-shape body
on Friday, January 15th, 2021 12:10 | by Andreas Ehweiner
Control of general FoxP expression patter (line 104y)
Expression of 104y (green) and FoxP (red)
There seems to be no colocalisation of 104y and FoxP.
Expression of 121y (green) and FoxP (red)
Overlap of 121y GFP expression with FoxP expression is the fan-shape body.
Problems with line c205, no GFP expression.
Category: Anatomy, Foxp | No Comments
ELAV-Gal4; TubGal80 results
on Monday, January 11th, 2021 12:28 | by Sarah-Lynn Stratil
A,B: KO in embyro stage
C: KO in larvae stage (Experiment 1: in 30 °C for 18 hours, Experiment 2: in 30 °C for 36 hours)
Category: Foxp | No Comments
ELAV-Gal4;Tub-Gal80: Temperature switch at embryo stage
on Tuesday, December 15th, 2020 2:34 | by Ottavia Palazzo
We phenocopied the results of the ELAV-Gal4 cross.
Now we are waiting for the results of the Temperature switch at the second instar larvae stage
Category: Foxp, Larve | No Comments
summary of the results of locomotion experiments on drosophila larvae so far and pcdf6 cloning scheme
on Monday, December 14th, 2020 1:06 | by Sarah-Lynn Stratil
method 1: food ring
method 2: one food patch
method 3: edge of petri dish
method 3.1: counting squares
0.1 cm grid under microscope, t= 1min
method 3.2: counting sqaures
| Crosses |
| ELAV-Gal4;TubGal80 x UAS-gRNA |
| ELAV-Gal4;TubGal80 x UAS-Cas9 |
| ELAV-Gal4;TubGal80 x UAS-Cas9- gRNA |
B: larvae-temperature scheme
25 °C until tested
| Crosses |
| ELAV-geneswitch x UAS-gRNA |
| ELAV-geneswitch x UAS-Cas9 |
| ELAV-geneswitch x UAS-Cas9- gRNA |
Category: Foxp | No Comments
FoxP knockout in adult flies
on | by Andreas Ehweiner
Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.
Category: flight, Foxp, Memory, Operant learning, operant self-learning, Uncategorized | No Comments
Crosses for conditional knockout
on Monday, December 7th, 2020 1:57 | by Sarah-Lynn Stratil
| Cross | Temp. | |
| ELAV-Gal4;TubGal80 x UAS-gRNA-Cas9 | 18 °C (day 1-x) | X |
| ELAV-Gal4;TubGal80 x UAS-gRNA | 18 °C (day 1-x) | X |
| ELAV-Gal4;TubGal80 x UAS- Cas9 | 18 °C (day 1-x) | X |
| ELAV-Gal4;TubGal80 x UAS-gRNA-Cas9 | 30 °C | |
| ELAV-Gal4;TubGal80 x UAS-gRNA | 30 °C (day 1-x) | X |
| ELAV-Gal4;TubGal80 x UAS- Cas9 | 30 °C |
Category: Foxp | No Comments