FoxP-KO at embryo stage

on Monday, March 30th, 2020 12:57 | by

The results again replicate the ones I had before.. FoxP flies where the temperature switch has been done for 12hrs during embyo stage can barely walk and surely can not fly.

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Potential Gal4-lines

on Monday, March 23rd, 2020 5:15 | by

Potential Gal4-lines matching parts of the FoxP expression.

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Search for neurons matching the FoxP expression pattern

I started looking at the FoxP expression pattern from Ottavia to see if i can identify the neurons.

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Ongoing experiments

on Sunday, March 22nd, 2020 10:50 | by

  1. PCB and ato crossed to GFP-RFP
  2. I crossed the tub-Gal80 again and I will temperature-induce in adulthood, then I will test at 5 days old, to see if I have any difference if compared to the ones tested at 3 days old
  3. Did the tub-Gal80 again with the induction during embryo, for andy to test together with his one
  4. Wrote almost all of the captions for the figures (exception made for the ones I need to finish experiments)
  5. Writing the results, I think I can manage to finish them for next monday.
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LexA+Gal4 larvae

on Friday, March 13th, 2020 3:30 | by

I tried the fast way but is not usable. Because without doing an additional crossing I have only GFP and RFP in heterozygosis. which gives a very weak signal. Furthermore I have to remove (flox) the dsRED that is there from the LexA insertion. Otherwise in the larva I see some cells that are red but that are due to the dsRED and not to the RFP. Which means other crossings. In total, to have this picture I need 5 crosses.
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Complete Buridan

on Tuesday, March 3rd, 2020 5:44 | by

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UASCas9;UASgFoxP X ElavG4;TubGal80

on Monday, March 2nd, 2020 1:33 | by

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Comparison FoxP and FoxP/+

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Supplementary figure S1 and S2

on Thursday, February 27th, 2020 11:13 | by

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Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46 | by

  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis
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