Test of 7 days old flies after GS

on Monday, February 15th, 2021 9:35 | by

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Local FoxP knok out GMR11F02-GAL4

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Preliminary test of 14 days old flies after GS

on Monday, January 25th, 2021 1:51 | by

With RU468 (2d), GSnsyb

No RU, GSnsyb

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GeneSwitch experiment

on Monday, January 18th, 2021 12:20 | by

larval locomotion
dFoxP knockout in embryonic stage

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FoxP in the fan-shape body

on Friday, January 15th, 2021 12:10 | by

Control of general FoxP expression patter (line 104y)

Expression of 104y (green) and FoxP (red)

There seems to be no colocalisation of 104y and FoxP.

Expression of 121y (green) and FoxP (red)

Overlap of 121y GFP expression with FoxP expression is the fan-shape body.

Problems with line c205, no GFP expression.

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ELAV-Gal4; TubGal80 results

on Monday, January 11th, 2021 12:28 | by

ELAV-Gal4; TubGal80: Temperature switch at embryo stage and larvae stage

A,B: KO in embyro stage
C: KO in larvae stage (Experiment 1: in 30 °C for 18 hours, Experiment 2: in 30 °C for 36 hours)

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ELAV-Gal4;Tub-Gal80: Temperature switch at embryo stage

on Tuesday, December 15th, 2020 2:34 | by

We phenocopied the results of the ELAV-Gal4 cross.

Now we are waiting for the results of the Temperature switch at the second instar larvae stage

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summary of the results of locomotion experiments on drosophila larvae so far and pcdf6 cloning scheme

on Monday, December 14th, 2020 1:06 | by

t=1 min
method 1: food ring
method 2: one food patch
method 3: edge of petri dish
0.25 cm grid, t=1 min
method 3.1: counting squares
0.1 cm grid under microscope, t= 1min
method 3.2: counting sqaures
Crosses
ELAV-Gal4;TubGal80 x UAS-gRNA
ELAV-Gal4;TubGal80 x UAS-Cas9
ELAV-Gal4;TubGal80 x UAS-Cas9- gRNA
each cross was made 3 times, to put in 18 °C, 25°C and 30°C
A: 25 °C larvae
B: larvae-temperature scheme
30 °C for two days
25 °C until tested

Crosses
ELAV-geneswitch x UAS-gRNA
ELAV-geneswitch x UAS-Cas9
ELAV-geneswitch x UAS-Cas9- gRNA
Crosses for GeneSwitch
pcdf6 cloning scheme

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FoxP knockout in adult flies

Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.

CD8GFP X nSyb-GS, 2d RU486
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Crosses for conditional knockout

on Monday, December 7th, 2020 1:57 | by

CrossTemp. 
ELAV-Gal4;TubGal80 x UAS-gRNA-Cas918 °C (day 1-x)X
ELAV-Gal4;TubGal80 x UAS-gRNA18 °C (day 1-x)X
ELAV-Gal4;TubGal80 x UAS- Cas918 °C (day 1-x)X
ELAV-Gal4;TubGal80 x UAS-gRNA-Cas930 °C 
ELAV-Gal4;TubGal80 x UAS-gRNA30 °C (day 1-x)X
ELAV-Gal4;TubGal80 x UAS- Cas930 °C 
crosses marked with an X are already in 18°C/30°C rooms.
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