Test of 7 days old flies after GS

on Monday, February 15th, 2021 9:35 | by

Print Friendly, PDF & Email

Local FoxP knok out GMR11F02-GAL4

Print Friendly, PDF & Email

Preliminary test of 14 days old flies after GS

on Monday, January 25th, 2021 1:51 | by

With RU468 (2d), GSnsyb

No RU, GSnsyb

Print Friendly, PDF & Email

FoxP knockout in adult flies

on Monday, December 14th, 2020 12:06 | by

Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.

CD8GFP X nSyb-GS, 2d RU486
Print Friendly, PDF & Email

Local FoxP knok out GMR20H5

on Monday, December 7th, 2020 9:55 | by

Print Friendly, PDF & Email

Local FoxP knock out

on Monday, November 16th, 2020 8:47 | by

Print Friendly, PDF & Email

Set-Up test

on Monday, November 9th, 2020 11:03 | by

Print Friendly, PDF & Email

New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

Print Friendly, PDF & Email

Testing of the repaired torque-meter

on Monday, October 12th, 2020 12:49 | by

I’ve started again to test the set-up after repairs. It does not seem to be perfectly stable ,with some drift in the positive direction. Most often, it seems that the amount of drifting would be fine for measuring.

Therefor I tried measuring some flies. A problem now is a very negative baseline, when the fly stops flying. Also the response for left and right seems not to be equal.

So now I’m trying to get the line close to 0, so that I have conclusive results if the set-up if usable at the current state.

2 examples of drift:

Print Friendly, PDF & Email

FoxP knock out in adult.

on Monday, September 14th, 2020 1:49 | by

The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)

Print Friendly, PDF & Email