Preliminary results from MBON screen and habit formation

on Monday, September 23rd, 2019 1:52 | by

The different MBON types and their averaged preference index for Test2/3 during isolated operant component in the flight simulator. Each letter corresponds to a randomized MBON subtype. All but one is being displayed due to low sample size.

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Inhibition of habit formation screening (MB011B)

on Wednesday, March 20th, 2019 1:45 | by

A flight performance test to see how the flies are behaving while flying. They have a slightly lower flight duration compared to the genetic controls but nothing that should impair them in the experiment.

The learning experiments are ongoing. I am doing n=15 for each line and each experiment, both Composite Control and also a Isolated Operant experiment. The Composite control experiment is done but I only got 13 flies for Isolated Operant. I do not want to preanalyze the data and will therefor post it when all the flies are done.

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Control experiments

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Learning paradigm with Wild Type Berlin flies

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Control experiments

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Been conducting a few control experiments to validate the “new” torque machine. Did a set of color learning and also isolated operant self learning. They do behave as expected. There is a small lumping together in the data the first period after the optomotor response in which the flies are in a neautral setting. It should be noted that it is a combined experiments of alternating right and left optomotor response. Also, the data in this figure is also a combination of the A/D converted “correctly” installed and “incorrectly”, i.e. inverted and reversed.

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Pushing software updates

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Johann Schmid and I have been working on getting updates to the torque meter software. Small changes but with significant increase in user friendliness.

● Inability to overwrite the data

● Progress bar and a time bar implemented

● It resets the pattern from one period to another. This is of critical importance as this enables one to do basically any kind of experiment on the machine

I have also gotten hold of a free version of LabView. I thought it could be a good idea that we could to small changes ourself to the software. However, my version is 2017 and Mr Schmid mentioned that he will be transferring to LabView 2019, and thereby retiring the 2017 version. A student version of labview is affordable, less than 50 Euros. It could be worth getting a legal licence of this software.


● The A/D converter now connects directly to the PCB. Only problem is that it is inverted, meaning that the signal from the torque machine gives a positive signal it is registered as a negative signal in the software. Mr Schmid is aware of this and will invert the signal in the software, rather than resoldering the PCB connection.

Updates to the DTS

● DTS is now also better compatible wit the new kind of data we are getting. A conflict occurred because of differences in pattern. An easy fix by just ignoring this parameter.

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Isolated operant component in the flight simulator

on Monday, January 21st, 2019 2:43 | by

Isolating operant component removes preference for left and right turning.

Each period is set to 120 seconds, meaning that the flies are getting a total of 8 minutes of training. The flies are performing two initial pre-tests, one test after 4 minutes of training and two final test periods. For the entire duration of the experiments flies are given a color indication if they are being punished or not as a result of left or right turning, this is a composite learning control. For the final test periods the colors have been removed, meaning that we are isolating the operant component. This is different from the previous tests that has been done where flies were not challenged with colors but just relied on their own behavior to determine which side is being punished. A removal of the helping colors resulted in a lack of preference for left- or right-turning maneuvers.

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Flight performance Ten-a cbd

on Monday, January 14th, 2019 2:54 | by

No differences in flight performance
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Measuring transmission spectra of color filters

on Monday, December 17th, 2018 1:32 | by

Measured with two different light sources. 

Dissection light

Light in torque meter

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17d flight simulator

on Monday, October 1st, 2018 2:58 | by

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17d

on Monday, September 10th, 2018 1:03 | by

Been working on 17d for the past month.

The experiments in the flight simulator. Self-learning performance indices in a two-minute test with the heat switched off after 4 and 8 minutes of training, indicated impairment of 17d-TNT flies.

The flies also showed clear impairments in their flight performance. To quantify this I assessed both possible alterations in their motor coordination (using climbing assay) as well as flight performance. The climbing assay relies on walking rather than flying. Both experiments show reduced ability of motor coordination and flight performance.

 

 

To confirm the specificity of the 17d-Gal4 fly I used the trans-tango flies.

Neuron, 96 (2017) 783-799. doi:10.1016/j.neuron.2017.10.011

 

The trans-tango is notorious for having a low expression in adult flies, which was also observed by me. The image is taking without any GFP-antibody.

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