Measuring transmission spectra of color filters

on Monday, December 17th, 2018 1:32 | by

Measured with two different light sources. 

Dissection light

Light in torque meter

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17d flight simulator

on Monday, October 1st, 2018 2:58 | by

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17d

on Monday, September 10th, 2018 1:03 | by

Been working on 17d for the past month.

The experiments in the flight simulator. Self-learning performance indices in a two-minute test with the heat switched off after 4 and 8 minutes of training, indicated impairment of 17d-TNT flies.

The flies also showed clear impairments in their flight performance. To quantify this I assessed both possible alterations in their motor coordination (using climbing assay) as well as flight performance. The climbing assay relies on walking rather than flying. Both experiments show reduced ability of motor coordination and flight performance.

 

 

To confirm the specificity of the 17d-Gal4 fly I used the trans-tango flies.

Neuron, 96 (2017) 783-799. doi:10.1016/j.neuron.2017.10.011

 

The trans-tango is notorious for having a low expression in adult flies, which was also observed by me. The image is taking without any GFP-antibody.

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Fussl shows numerical difference in operant self learning

on Tuesday, August 7th, 2018 2:49 | by

Fussl was crossed with either Stinger (ctrl) or a UAS-TNT line to block the synaptric transmission of the Fussl positive neurons. A third construct was used but did not yield any data due to difficulties with their flight performance. The Fussl-Stinger along with Fussl-TNT flies do also show difficulties in flying. These differences will be assessed.

The experiment was done as a pilot experiment before doing a larger scale.

The data is a bit inconsistent but shows a positive and reassuring numerical difference. The control is a bit lower than expected, compared to WTB flies (showing usually a PI 0f 0.6). The flies have a slightly different background than wtb flies and have pale orange eyes (still no apparent impairments in vision). Further experiments will be conducted before proceeding with a larger sample size of the flies.

 

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Assessing the difficulties in self-learning for FoxP flies

on Monday, July 30th, 2018 1:52 | by

FoxP3955 flies were raised and compared to normal WTB flies. Reportedly, the Foxp mutants have a reduced flight performance as their total flight duration is decreased. This was also something I experienced. The problem seemed to be greater due to the heat in the flight simulator room, initial temperature was 27°C but increased to close to 30°C. I had troubles getting a large sample size enough (same number of Foxp and wtb were loaded into the flight simulator), heat-shock proteins and other stress-related behavior might be an issue. The genotype of the flies were known during the hooking of the flies but was later on concelead and flies were randomly distributed.  

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Running the flight simulator analysis script

on Monday, July 2nd, 2018 1:55 | by

Wildtype flies and flight simulator Part 2

on Monday, June 11th, 2018 1:29 | by

Replicated wild type berlin flies in the flight simulator

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Wildtype flies and flight simulator

on Monday, June 4th, 2018 9:18 | by

The results from wiltype Berlin flies in the flight simulator.

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Sathish scripts in my hands are reproducing results

on Friday, May 18th, 2018 3:34 | by

 

 

This is a picture of the supplemental figure from Maye et al. 2007

 

Below the results from the sathish scripts running on the data from Maye et al. 2007. It matches, so Sathish scripts in my hands work fine

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Confocal images and boxplots from my results in strokelitude

on Tuesday, May 15th, 2018 12:26 | by

Confocal image MAX stack of one of the brains at 20x

 

 

and at 40x

 

In this link we have a video of a 3D stainning pattern          zoomed_CC

 

In addition I add here teh boxplots from the final results of the Ping Pong ball setup with these experiments

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