Simon Benmaamar

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Rover/Sitter Density Experiment

on Monday, January 18th, 2021 1:30

1. Food Patch Experiment

Control

There was a significant difference between the groups (t-test, p =0.01274)

Crowded

There was no significant difference between the groups (t-test, p = 0.2284)

2. Tracking

60 seconds

Control

There was a significant difference between the groups (t-test, p =0.000162)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.07565)

Crowded

45 seconds

Control

There was a significant difference between the groups (t-test, p =0.001252)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.2159)

Crowded

Planned experiments

on Monday, December 21st, 2020 11:37

Latest food patch experiments on Rover/Sitter larvae

on Sunday, December 20th, 2020 5:31

Food Patch Experiment

For Control larvae from the 11.12.2020 and 14.12.2020. There is nearly no difference between both groups.

Time of first movement function

Additional function to plot the time when the larva starts moving. The Data from last week was used

Fly food analysis

on Monday, December 14th, 2020 11:54

Further testing of the For Control Group

on

Food patch experiment

Barplot of the For Control Group with additional n=4 .

Distance tracking

The larvae were filmed for one minute. Larvae that reached the edge of the petri dish before one minute were discarded.
Video recording was started as soon as the larvae where placed on the agar plate.
Same data cut after 30 s.

New experiments on Rover/Sitter larvae

on Monday, December 7th, 2020 12:29

Testing new tracking software: Kinovea

Barplot of the distance of the Canton-S WT larvae of last week. There is no big difference compared to the tracking with Biotrackr.

Food patch experiments on old Rover/Sitter larvae

ForS and ForR larvae raised on new food. There is a bigger difference between the groups.

Distance tracking of old Rover/Sitter larvae

The larvae were filmed for one minute. Larvae that reached the edge of the petri dish before one minute were discarded.
Video recording was started as soon as the larvae started moving.

Distance tracking of larvae

on Monday, November 30th, 2020 11:57

Testing of the tracking and R Code

1) Small paper ball to simulate a larva, attached to a black string, and moved 2 cm by hand. 2) Measuring the pixel distance of a known real life distance (1 cm). 3) Plot of x and y coordinates shows trajectory of the object. 4) Bar plot of the real life distance shows that tracking and the R code worked .

Distance tracking of Canton-S wild type larvae

1) Larva on agar plate dyed with activated charcoal 2) Barplot of the distance of the Canton-S WT larvae

Problem finding the video time

Output of the csv file of the BioTracker.

Further experiments on larval locomotion using Canton-S wild type flies

on Sunday, November 22nd, 2020 10:33

All experiments were done using 1 mm graph paper and a microscope to count the boxes more precisely.

Tracking of the larvae using Ctrax

Agar plate dyed with activated charcoal (2 g charcoal/ 1 l H2O)
Tracking with Ctrax software using the Burridan camera setup.
Category: Larve | No Comments

Experiments on larval locomotion using Canton-S wild type flies

on Friday, November 13th, 2020 11:07

Time Experiments

One larva with a time exceeding 20 minutes was not considered.
Two larvae with a time exceeding 20 minutes were not considered.

Distance Experiments

Experiment 4: Graph Paper

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New evaluation of the Rover/Sitter larvae

on Thursday, November 5th, 2020 12:34
Control Group 1 has a higher density of larvae compared to Control Group 2.

Percentage of Sitter and Rover larvae, who stayed on the first food patch after 20 minutes.

Course Evaluation