Ottavia PalazzoView Profile
First try for GABA antibody on FoxPisoB-Gal4 adult brain. The reaction is not optimal, there is a lot of background and the signal is too strong. We will have to try again changing conditions in order to be sure of any colocalization.
We also have found some FoxP-all_Isoforms-Gal4 homozygous, we thus wanted to see if there was any macro problem in the overall structure of the brain, but the structure looks fairly normal
I did the WB again in order to have a better and clearer image, additionally I made a coomassie staining and a silver staining in order to see if there is any contamination in the purified FoxP-IsoB protein
I also made a WB on Drosophila brains using the mouse serum. The signal is not clean but we can see a band of the appropriate size (60 kDa)
The sera of the mice (expecially the one immunized with URE-10 peptide) show titer against FoxP recombinant protein. In september we can proceed with the hybridoma using the mouse immunized with ure-10
Immunostaining against ELAV and BruchPilot in FoxP-IsoB-Gal4 flies (ELAV: 20x and 40x, BruchPilot 20x)
The ELISA titer for the FoxP antibody production is not changing with the number of the immunizations (on the contrary it looks slightly reduced). The titer of the peptide URE-10 looks better then the URE-9. We will proced with a fifth immunization.
Here the complete graph for URE-10 with the pre-bloood and the first immunization