Ottavia Palazzo

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Larvae FoxPB-Gal4 with ELAV or REPO

on Friday, September 14th, 2018 1:05

Larvae ELAV (20x and 40x)

Adults (20x and 40x)

Larvae REPO (20x and 40x)

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First try for GABA antibody + FoxP-all_isoforms-Gal4

on Sunday, September 2nd, 2018 12:41

UNIVERSITY PART

First try for GABA antibody on FoxPisoB-Gal4 adult brain. The reaction is not optimal, there is a lot of background and the signal is too strong. We will have to try again changing conditions in order to be sure of any colocalization.

We also have found some FoxP-all_Isoforms-Gal4 homozygous, we thus wanted to see if there was any macro problem in the overall structure of the brain, but the structure looks fairly normal

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Larvae FoxPisoB-Gal4

on

UNIVERSITY PART

Larvae FoxPisoB-Gal4 + Bruch Pilot (20x and 40x)

 

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Western Blot FoxP-IsoB

on Friday, August 24th, 2018 2:19

KLINIKUM PART

I did the WB again in order to have a better and clearer image, additionally I made a coomassie staining and a silver staining in order to see if there is any contamination in the purified FoxP-IsoB protein

I also made a WB on Drosophila brains using the mouse serum. The signal is not clean but we can see a band of the appropriate size (60 kDa)

 

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WB and New clone for FoxP all isoform

on Thursday, July 19th, 2018 4:24

KLINIKUM PART

The serum from the forth injection recognize the FoxP recombinant protein. The band has the appropriate size

UNIVERSITY PART

I have exchanged the Hom1 domain that had a frameshift. Ready to inject again

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Titer against FoxP recombinant protein and Immuno

on Thursday, July 5th, 2018 4:51

KLINIKUM PART

The sera of the mice (expecially the one immunized with URE-10 peptide) show titer against FoxP recombinant protein. In september we can proceed with the hybridoma using the mouse immunized with ure-10

UNIVERSITY PART

Immunostaining against ELAV and BruchPilot in FoxP-IsoB-Gal4 flies (ELAV: 20x and 40x, BruchPilot 20x)

BruchPilot alone

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Cell counting of FoxP-IsoB in stinger staining

on Monday, July 2nd, 2018 1:45

Cell counting of 2 adult brains  for the Stinger staining in FoxP-IsoB-Gal4 flies. The expression is symmetrical and similar in the 2 brains. I am planning to do some more staining and counting at least 10 brains that I will compare with the FoxP all isoforms-Gal4

 

 

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FoxPE-Gal4 + REPO and BruchPilot

on Sunday, June 24th, 2018 5:42

Stinger + REPO (20x and 40x): Visualization of gliacells (anti-Repo) and GFP-FoxPE expressing Neurons at 20x and 40x

CD8-GFP + BruchPilot

 

BruchPilot alone

PCR Amplification of HomI and HomII: Amplification of the Inserts for the Knock-Out of FoxPE

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Crosses for isogenize FoxP-IsoB-Gal4 line

on Tuesday, June 19th, 2018 12:13

ELISA titer not changing

on Sunday, June 17th, 2018 8:00

KLINIKUM PART

The ELISA titer for the FoxP antibody production is not changing with the number of the immunizations (on the contrary it looks slightly reduced). The titer of the peptide URE-10 looks better then the URE-9. We will proced with a fifth immunization.

Here the complete graph for URE-10 with the pre-bloood and the first immunization

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