Ottavia Palazzo

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FoxP-KO

on Sunday, November 25th, 2018 3:46

FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough

 

 

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Hybridoma (Fusion) 1st screen

on Wednesday, October 24th, 2018 5:06

This picture shows all of the positive clones in the ELISA from the 1st screen

While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA

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FoxPB adult cell count

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I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis

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FoxP-KO or WTB?

on Wednesday, October 17th, 2018 4:04

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

 

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

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63x picture of 2 of the FoxPB-Gal4 positive clusters

on Sunday, October 7th, 2018 6:11

The dorsal cluster neurons are really evident and with a typical organization

From Hassam et al., 2000

Protocerebral bridge in FoxP-KO flies

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The more brains I see, the more I am convinced that the problem is not about my dissection

MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)

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this result is still to be verified by deleting the red fluorescent eyes to the flies

Counting of FoxP-isoB-Gal4 positive cells

on Wednesday, September 26th, 2018 10:19

Counting of FoxP-isoB-Gal4 positive cells in adult and larvae brains (no VNC). Five brains counted for each age

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Tries with IMARIS

on Friday, September 21st, 2018 2:07

Me and Anders spent some time at the imaris trying to get confidence with the software. We managed to do volume rendering and we counted the FoxP-isoB positive cells

  

the count resulted really similar to the one I´ve done by eyes:

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Protocerebral Bridge in FoxP-Gal4 and in FoxP-KO

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