Ottavia Palazzo

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WB and New clone for FoxP all isoform

on Thursday, July 19th, 2018 4:24


The serum from the forth injection recognize the FoxP recombinant protein. The band has the appropriate size


I have exchanged the Hom1 domain that had a frameshift. Ready to inject again

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Titer against FoxP recombinant protein and Immuno

on Thursday, July 5th, 2018 4:51


The sera of the mice (expecially the one immunized with URE-10 peptide) show titer against FoxP recombinant protein. In september we can proceed with the hybridoma using the mouse immunized with ure-10


Immunostaining against ELAV and BruchPilot in FoxP-IsoB-Gal4 flies (ELAV: 20x and 40x, BruchPilot 20x)

BruchPilot alone

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Cell counting of FoxP-IsoB in stinger staining

on Monday, July 2nd, 2018 1:45

Cell counting of 2 adult brains  for the Stinger staining in FoxP-IsoB-Gal4 flies. The expression is symmetrical and similar in the 2 brains. I am planning to do some more staining and counting at least 10 brains that I will compare with the FoxP all isoforms-Gal4



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FoxPE-Gal4 + REPO and BruchPilot

on Sunday, June 24th, 2018 5:42

Stinger + REPO (20x and 40x): Visualization of gliacells (anti-Repo) and GFP-FoxPE expressing Neurons at 20x and 40x

CD8-GFP + BruchPilot


BruchPilot alone

PCR Amplification of HomI and HomII: Amplification of the Inserts for the Knock-Out of FoxPE

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Crosses for isogenize FoxP-IsoB-Gal4 line

on Tuesday, June 19th, 2018 12:13

ELISA titer not changing

on Sunday, June 17th, 2018 8:00


The ELISA titer for the FoxP antibody production is not changing with the number of the immunizations (on the contrary it looks slightly reduced). The titer of the peptide URE-10 looks better then the URE-9. We will proced with a fifth immunization.

Here the complete graph for URE-10 with the pre-bloood and the first immunization

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Confocal images (FoxP-IsoB-Gal4 x CD8-GFP)

on Thursday, June 14th, 2018 3:27

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CD8-GFP x FoxP-IsoB_Gal4

on Friday, June 8th, 2018 11:47

Not confocal immages (going to the confocal this week)

The construct I am analyzing is the FoxP-IsoB_Gal4, so with the Gal4 inserted in the 8th exon


Adult VNC

The Gal4 line is working

on Wednesday, May 30th, 2018 4:58

Here some preliminary pictures (not confocal) of the FoxP.IsoB-Gal4 line crossed with a Stinger line (GFP expression only in the nucleus)

and with a 6xCD8-GFP (a little bit massacred), this week I will do them again

And here´s the whole larva for the 6xCD8-GFP

I will try to go to the confocal as soon as possible

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Schemes for FoxP and FoxP isoB constructs

on Friday, May 18th, 2018 3:31

This is the technique we are using to create the non conditional constructs: HDR using CRISPR/Cas9 (S. J. Gratz et al., 2014)


and these are the schemes of the constructs we are creating

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