Ottavia Palazzo

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New figure?

on Tuesday, September 8th, 2020 11:16
Category: Foxp | No Comments

August

on Thursday, August 27th, 2020 1:46
  • Halfway in analyzing the Buridan data altogether (not in two batches)
  • Maxiprepped plasmid for making FoxP protein at the klinikum
  • Writing the thesis a little bit
  • bought Fas-II antibody

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  • data 1): FoxP-iB Heterozygous/Homozygous comparison with Stinger-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can not detect any difference between homozygous and heterozygous mutants. I also counted cells in IMARIS and I detected no difference in number or distribution.

  • data 2): FoxP-iB Heterozygous/Homozygous comparison with CD8-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can see some differences but i do not know how to quantify/explain them. Also I am not sure if it is a problem of dissection/mounting. I am not convinced.

  • data 3): nc82 staining on homo/hetero FoxP-iB. I do not see differences

Problem: I haven’t managed yet to make the FasII antibody work:

I have used a 1:10 concentration of primary antibody for one night and a 1:100 concentration of secondary antibody for 4 hours. I will ask someone

In the picture I have increased the gain a lot just to be sure. I could not see anything

Category: Foxp | No Comments

Pupae TS increased N

on Monday, June 22nd, 2020 10:34

Tdc2-Gal4 + FoxP-LexA in VNC

on Thursday, June 18th, 2020 4:16
Red: FoxP
Green: Tdc2
No colocalization also in the VNC
Category: Foxp | No Comments

C380-increased N

on Wednesday, June 17th, 2020 9:49

Last week

on Monday, June 15th, 2020 12:52
  1. Crossed the flies for the course
  2. Almost finished the Buridan for C380
  3. Worked on the manuscript and supplemetary figures
  4. Done the FoxP reaction on induced embryo again because I was afraid after the different results Andi had last time
I fortunately still see nothing
Category: Foxp | No Comments

ok107-increased N

on Monday, June 8th, 2020 10:32

qPCR-Joel

on Wednesday, June 3rd, 2020 1:43
I did the graph on Joel’s data
Category: Foxp | No Comments

Last week

on Monday, June 1st, 2020 10:56
  1. Made crosses for pupae TS
  2. Made cross for Tdc2-Gal4 and FoxP-LexA to see the VNC (I already had last week the pictures of the CB)
  3. Made crosses for ok107
  4. Made crosses for C380

After all those crosses will hatch I will have finished with the Buridan for now.

Category: Foxp | No Comments

Tub-Gal80 Adult increased N

on

Did it again and now looks fine :)