Ottavia Palazzo

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FoxPB+ cells in adult flies (almost) finished

on Monday, December 17th, 2018 11:59
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Homo and Hetero CD8-GFP;FoxPB-G4 Part2

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I kept analyzing brains and the phenotype is conserved

Homo and Hetero CD8-GFP;FoxPB-G4

on Monday, December 10th, 2018 10:11

Age and sex dependent FoxPB-Gal4 line count

on Thursday, December 6th, 2018 11:24
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FoxPB-IsoB + p-Smad

on Tuesday, December 4th, 2018 4:08
a finer analysis shows that a big percentage of FoxPB cells in the VNC is also positive to p-SMAD, while FoxPB cells in the central brain are not

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Age and sex related FoxPB-Gal4 flies count

on Monday, December 3rd, 2018 11:44
I am still waiting for the flies to age in order to have a count also for 6 days and 10 days

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FoxPB-Gal4 + p-smad (motorneurons)

on Sunday, December 2nd, 2018 3:29
The reaction is not optimal, but it looks like there are some double positive cells, and this indicate that some neurons could be motorneurons. To identify a % more and better reactions should be done

Entire larva:

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FoxP-KO

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FoxP-KO

WT (from random papers)

FoxP-KO

on Sunday, November 25th, 2018 3:46
FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough

 

 

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Hybridoma (Fusion) 1st screen

on Wednesday, October 24th, 2018 5:06

This picture shows all of the positive clones in the ELISA from the 1st screen

While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA

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