Ottavia Palazzo

View Profile

FoxP-KO at embryo stage

on Monday, March 30th, 2020 12:57

The results again replicate the ones I had before.. FoxP flies where the temperature switch has been done for 12hrs during embyo stage can barely walk and surely can not fly.

Category: Foxp | No Comments

Ongoing experiments

on Sunday, March 22nd, 2020 10:50
  1. PCB and ato crossed to GFP-RFP
  2. I crossed the tub-Gal80 again and I will temperature-induce in adulthood, then I will test at 5 days old, to see if I have any difference if compared to the ones tested at 3 days old
  3. Did the tub-Gal80 again with the induction during embryo, for andy to test together with his one
  4. Wrote almost all of the captions for the figures (exception made for the ones I need to finish experiments)
  5. Writing the results, I think I can manage to finish them for next monday.
Category: Foxp | No Comments

LexA+Gal4 larvae

on Friday, March 13th, 2020 3:30
I tried the fast way but is not usable. Because without doing an additional crossing I have only GFP and RFP in heterozygosis. which gives a very weak signal. Furthermore I have to remove (flox) the dsRED that is there from the LexA insertion. Otherwise in the larva I see some cells that are red but that are due to the dsRED and not to the RFP. Which means other crossings. In total, to have this picture I need 5 crosses.
Category: Foxp | 2 Comments

Complete Buridan

on Tuesday, March 3rd, 2020 5:44

Supplementary figure S1 and S2

on Thursday, February 27th, 2020 11:13
Category: Foxp | No Comments

Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46
  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis

Graphs new look

on Wednesday, February 19th, 2020 11:17
Category: Foxp | No Comments

LexA construct qPCR

on Wednesday, February 12th, 2020 3:35
First graph: FoxP-iB-Gal4
Second graph: FoxP-LexA
Category: Foxp | No Comments

Additional ELAV-Gal4>UAS-gRNA control

on Thursday, February 6th, 2020 5:23

Last week

on Friday, January 31st, 2020 3:50
  1. extracted RNA and done cDNA from it from WTB flies and Hetero flies (Homo were too few). Doing the qPCR with those this week
  2. Inserted GFP in homozygosis in FoxP-KO flies
  3. Crossed Elav-Gal4 x UAS-gRNA-FoxP and Elav-Gal4;Tub-Gal80ts x UAS-gRNA-FoxP (left at 30 degrees) as controls for buridan
  4. Crossed protocerebral bridge line for buridan
  5. Finished the presentation for thursday
  6. Writing material and methods for the thesis
Category: Foxp | No Comments