Ottavia Palazzo

View Profile

FoxP-KO or WTB?

on Wednesday, October 17th, 2018 4:04

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

 

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

Category: Foxp | No Comments

63x picture of 2 of the FoxPB-Gal4 positive clusters

on Sunday, October 7th, 2018 6:11

The dorsal cluster neurons are really evident and with a typical organization

From Hassam et al., 2000

Protocerebral bridge in FoxP-KO flies

on

The more brains I see, the more I am convinced that the problem is not about my dissection

MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)

on

this result is still to be verified by deleting the red fluorescent eyes to the flies

Counting of FoxP-isoB-Gal4 positive cells

on Wednesday, September 26th, 2018 10:19

Counting of FoxP-isoB-Gal4 positive cells in adult and larvae brains (no VNC). Five brains counted for each age

Category: Foxp | No Comments

Tries with IMARIS

on Friday, September 21st, 2018 2:07

Me and Anders spent some time at the imaris trying to get confidence with the software. We managed to do volume rendering and we counted the FoxP-isoB positive cells

  

the count resulted really similar to the one I´ve done by eyes:

Category: Foxp | No Comments

Protocerebral Bridge in FoxP-Gal4 and in FoxP-KO

on

Category: Foxp | No Comments

Protocerebral bridge in FoxP mutated flies

on Friday, September 14th, 2018 1:22

The first 3 images show the protocerebral bridge in FoxP mutated flies. In the second line the first picture shows the protocerebral bridge in a FoxP isoB-Gal4 fly. The last image is taken and modifies from the Virtual Fly Brain

Category: Foxp | No Comments

Larvae FoxPB-Gal4 with ELAV or REPO

on

Larvae ELAV (20x and 40x)

Adults (20x and 40x)

Larvae REPO (20x and 40x)

Category: Foxp | No Comments

First try for GABA antibody + FoxP-all_isoforms-Gal4

on Sunday, September 2nd, 2018 12:41

UNIVERSITY PART

First try for GABA antibody on FoxPisoB-Gal4 adult brain. The reaction is not optimal, there is a lot of background and the signal is too strong. We will have to try again changing conditions in order to be sure of any colocalization.

We also have found some FoxP-all_Isoforms-Gal4 homozygous, we thus wanted to see if there was any macro problem in the overall structure of the brain, but the structure looks fairly normal

Category: Foxp | No Comments