Ottavia Palazzo

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FoxP-LexA – cell counting (IMARIS)

on Monday, July 22nd, 2019 10:38

3rd instar larvae: 406 (n=2)

Adult: 1430 (n=2) (VNC excluded)

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FoxP-LexA + nc82

on Thursday, July 18th, 2019 10:45
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FoxPB-Gal4+nc82

on Saturday, July 13th, 2019 3:28
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FoxP-LexA x GFP Larvae

on Friday, July 12th, 2019 2:40
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Tub-Gal80 flies with HS at embryo stage + statistic on old results

on Friday, July 5th, 2019 11:44
The combination of heat and the FoxP KO is making the flies too sick to develop properly. This leads to difficulties in growing. Even though I can not test for behaviour it´s a good result because it means that the Tub-Gal80 line is working, and it validates my other results

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FoxP conditional KO – immunohistochemistry with FoxP antibody

on Friday, June 14th, 2019 10:54
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Cell count on CB and VNC

on Friday, May 17th, 2019 12:00

The previous cell count was done considering only the central brain. Here I included the VNC in order to have a more complete information

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ChAT on adults

on

Unfortunately it looks like it is impossible for me to recognize cell bodies. I can only see neuropil, therefore I can not look for colocalizations.

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LexA construct

on Monday, May 6th, 2019 11:05

After the second sequencing it shows no frameshift, so the construct is ready to be injected (I took an appointment with Renate and we will inject the 28th of may)

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ChAT on Larvae

on
Colocalizations with FoxPB in both VNC and central brain
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