Bianca BirkView Profile
Figure 1 shows the results of the tbh-subgroups screen, tested at RT and 24 h later at 32°C.
All genotypes with n=8, expect tbh(39942) x Shi^TS (n=4).
Figure 1 shows the newest results of the screen of the tbh-subgroups.
In addition I dissected brains of these flies.
As a next step I’ll compare my “stainings” to the ones from flybase.
I started testing some more tbh-GAL4-subgroups. In Fig. 1 you see all tbh-subgroups, the last 5 in the graph are the newest trials (n=2).
Moreover I dissected some more brains.
In Fig 1 you see the results of the tbh – subgroups screen.
Furthermore I plotted them in a dot plot. (Fig. 2 and Fig. 3)
Moreover I dissected some brains which I crossed to GFP (nucl.)
Fig4. tbh-48331 x GFP (nucl)
These are the newest results of the tbh-subgroups screen.
‘Staining’ of tbh subgroup 46970
I crossed tbh-46970 to GFP (nucl), dissected the brains and mounted them (No antibodies were used).
I tested some more tbh-GAL4 subgroups. Figure 1 shows the newest results of the screen.
Because of the weird results, we decided to make crossbreedings the other way around. For the last crossbreedings I took virgins of the UAS-Shi^TS and males from the tbh-GAL4 subgroups, now I take virgins of the tbh-GAL4s. So in two weeks the first crossbreedings will hatch and I will test them.
I tested some more tbh-GAL4 crossed to shibire^TS. These are the newest results of the screen.
I continue testing the tbh-Gal4 subgroups and tbh-lexA.
Figure 1 shows the newest results of tbh-lexA (n=6)
Figure 2 shows the newest results of tbh-Gal4 (tbh48331, tbh46970 n=3, tbh39942 n=1, rest with a n of 2)
I practiced the dissections of brains for stainings. So I fixed the flies for one hour in PFA 4% , then I dissected the brains, washed them a few times with PBST and after it I mount them with vectashield.
Figure 3 shows a picture of a brain of a Cha-Gal80;TH-Gal4 x GFP (nucl.) flie.
I want to know which octopaminergic neurons are involved in the phototactic switch after wing clipping. So I started with a screen for some subgroubs of tbh-GAL 4. After testing the flies in RT with a T-Maze, I tested the same flies again in 32 degree. I expect, that wingless flies go to the light, so the Choice Index should increase. The Figure 1 shows the results of only 2 experiments (39942-tbh-GAL4 only one experiment).