E. Axel GorostizaView Profile
I recently combined the two TbH-lexA lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.
TDC2>GFP and anti-TβH (Scholz’s Lab)
TDC2>GFP (Gerber’s Lab)
anti-TDC2 (Goodwin’s Lab)
After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.
I have been doing crosses in order to establish different lines that will allow me to study the neurons behind the photopreference shift. Here I show the first dissections I did to test some of these lines.
Dopaminergic clusters (Left: posterior, Right: anterior)
TH-C’ from the original publication (Top: anterior, Bottom: posterior)