E. Axel Gorostiza

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Starting with optogenetics in photopreference

on Monday, June 19th, 2017 2:58

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.

 

Problems solved? (photopreference)

on

These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C

 

Some controls for the photopreference Troubleshooting

on Monday, June 12th, 2017 2:53

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.

 

DA clusters and Photopreference

on Monday, May 22nd, 2017 12:17

Here is an update on the evaluation of the DA clusters in Photopreference.

 

Update on DA & photopreference

on Monday, April 24th, 2017 1:55

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

Photopreference shift and DA (new and old GAL4s)

on Monday, April 3rd, 2017 1:56

Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.

 

Behavioral Test on DA-GAL4s

on Monday, March 27th, 2017 2:06

I am currently testing different dopaminergic GAL4s in order to validate previous results from my initial screening. Here I can find the results for one of those GAL4s

 

 

TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5

TH-GAL4 subgroups (Anatomy)

on Monday, January 30th, 2017 11:42

I have been doing crosses in order to establish different lines that will allow me to study the neurons behind the photopreference shift. Here I show the first dissections I did to test some of these lines.

Dopaminergic clusters (Left: posterior, Right: anterior)

My dissections

TH-C’ from the original publication (Top: anterior, Bottom: posterior)