E. Axel Gorostiza

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Optogenetics and photopreference

on Monday, July 24th, 2017 10:39

I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.

Blue: only blue light.

Red: Blue light + pulsed red light.

 

 

I think Chrimson is already activated under my blue light condition.

 

Photopreference and optogenetics

on Monday, July 17th, 2017 2:57

Photopreference and Speed

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This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.

 

Optogenetics in Photopreference (Pretests)

on Monday, July 10th, 2017 12:31

On this opportunity, I changed the size of the tubes in order to see if that has a impact on the proportion of flies that ended in the elevator.

 

Optogenetics in Photopreference

on Monday, July 3rd, 2017 1:26

Here, I present you the results of using Blue light for photopreference and adding a pulsed Red light. These experiments are controls required for using Optogenetics to study Photopreference. I used the Blue light at different intensities, and calculated the Choice Index (CI) and the proportion of flies in each tube of the T-Maze (White: Bright, Black: Dark, Grey: Elevator).

 

 

Starting with optogenetics in photopreference

on Monday, June 19th, 2017 2:58

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.

 

Problems solved? (photopreference)

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These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C

 

Some controls for the photopreference Troubleshooting

on Monday, June 12th, 2017 2:53

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.

 

DA clusters and Photopreference

on Monday, May 22nd, 2017 12:17

Here is an update on the evaluation of the DA clusters in Photopreference.

 

Update on DA & photopreference

on Monday, April 24th, 2017 1:55

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.