E. Axel Gorostiza

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Optogenetics and photopreference

on Monday, July 24th, 2017 10:39

I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.

Blue: only blue light.

Red: Blue light + pulsed red light.



I think Chrimson is already activated under my blue light condition.


Photopreference and optogenetics

on Monday, July 17th, 2017 2:57

Photopreference and Speed


This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.


Optogenetics in Photopreference (Pretests)

on Monday, July 10th, 2017 12:31

On this opportunity, I changed the size of the tubes in order to see if that has a impact on the proportion of flies that ended in the elevator.


Optogenetics in Photopreference

on Monday, July 3rd, 2017 1:26

Here, I present you the results of using Blue light for photopreference and adding a pulsed Red light. These experiments are controls required for using Optogenetics to study Photopreference. I used the Blue light at different intensities, and calculated the Choice Index (CI) and the proportion of flies in each tube of the T-Maze (White: Bright, Black: Dark, Grey: Elevator).



Starting with optogenetics in photopreference

on Monday, June 19th, 2017 2:58

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.


Problems solved? (photopreference)


These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C


Some controls for the photopreference Troubleshooting

on Monday, June 12th, 2017 2:53

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.


DA clusters and Photopreference

on Monday, May 22nd, 2017 12:17

Here is an update on the evaluation of the DA clusters in Photopreference.


Update on DA & photopreference

on Monday, April 24th, 2017 1:55

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.