E. Axel Gorostiza

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Update on DA & photopreference

on Monday, April 24th, 2017 1:55

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

Photopreference shift and DA (new and old GAL4s)

on Monday, April 3rd, 2017 1:56

Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.

 

Behavioral Test on DA-GAL4s

on Monday, March 27th, 2017 2:06

I am currently testing different dopaminergic GAL4s in order to validate previous results from my initial screening. Here I can find the results for one of those GAL4s

 

 

TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5

TH-GAL4 subgroups (Anatomy)

on Monday, January 30th, 2017 11:42

I have been doing crosses in order to establish different lines that will allow me to study the neurons behind the photopreference shift. Here I show the first dissections I did to test some of these lines.

Dopaminergic clusters (Left: posterior, Right: anterior)

My dissections

TH-C’ from the original publication (Top: anterior, Bottom: posterior)

Short update on DA in photopreference

on Monday, January 23rd, 2017 2:16

A few months ago I finished my first screen for DA-neuronal populations relevant in the photopreference shift. I tried a small collection of TH-Gal4s with interesting results that you could see here. To validate those results, I am currently searching for other Gal4s labeling the same groups of neurons. Here you can see the outcome of the first experiments for one of those lines.

OA in photopreference (other GAL4s)

on Monday, January 16th, 2017 2:50

Trying to define subsets of neurons related to the photopreference shift, I started to test the different OA and DA drivers available. Here you can see two OA-GAL4s used in Burke et al 2012:

My results:

Individual Choices in Benzer.

on Monday, November 7th, 2016 2:46

The following figure depict the PI for flies tested in a Benzer CC paradigm, and the PI for the subgroups generated at the end of the first test in a re-test. Additionally, I calculated the PI expected for flies in groups 5-4 (green) and 3-0 (yellow) if they would choose the same tubes in the re-test.

 

benzer-subgrous-3h-later-pi-theoretical-subgroups

Figures for SfN (Individual choices in Benzer and T-maze paradigms)

on Monday, October 31st, 2016 2:55

Since SfN is around the corner, I am preparing the figures for my poster. This year the title is “Do flies in groups make individual choices?”, and contains the work that was done by Isabelle during her Bachelor thesis.

I redesigned the figures, and here are the most important ones.

Amount of flies in T-Maze and how it affects flies’ choices/distribution in tubes:

amount-of-flies-t-maze

 

Subgroups obtained after testing an initial population in the T-Maze:

3h later

t-maze-subgrous-3h-later

24h later

t-maze-subgrous-24h-later

Amount of flies in Benzer’s paradigm and how it affects flies’ choices/distribution in tubes:

amount-of-flies-benzer

Subgroups obtained after testing an initial population in Benzer’s paradigm:

3h later

benzer-subgrous-3h-later

24h later

benzer-subgrous-24h-later

All Benzer subgroups (unfinished figure):

benzer-all-groups