Progresses in antibody production

on Friday, April 6th, 2018 12:09 | by Ottavia Palazzo

KLINIKUM PART

After 2 immunization on the mice we made an ELISA test in order to see if there is an immunogenic response in the serum. There is a response for both the peptides, the only problem is that is not really specific: the mice immunized with one peptide (URE-9) show a response also for the other peptide (URE-10), even if slightly lower, and viceversa.

Also the response is not great enough, therefore a 3rd immunization is required.

 

We also finally have the recombinant protein (FoxP IsoB-FLAG). Here´s the picture of the western blot with the band of the appropriate size (60 kD) on the right. On the left is the same band from the cell lysate.

Print Friendly, PDF & Email

Category: Foxp

Leave a Reply

Your email address will not be published. Required fields are marked *