As a control for my T-Maze experiments I decided to test if treated flies were able to fly at the same conditions they were tested in the T-Maze (32°C).

As in the T-Maze experiments, I put the flies 15 min at 32°C before testing them at the same temperature. The, I measure the duration of the first spontaeous flight (Blue). If within 5min the fly doesn’t start to flight, I gently remove the steroform ball from the legs (Red), simulating a takeoff.

Details from 0 s to 200 s.