on Wednesday, October 7th, 2015 5:44 | by Isabelle Steymans
In the previous experiment we saw that testing tubes 5 and 4 together show almost the same distribution than the first round of the benzer paradigm used to split them, however the tubes 3 to 0 together showed a big change. I decided to test single tubes, to see how big the difference between those subgroups actually is. I started by doing the Benzer paradigm to split the group and then I did the Benzer paradigm a second time only using the flies from a particular tube. I wanted to take both extremes, but in the tubes 0-2 there were not many flies. Therefore, I decided to test tubes 5 and 3. Considering that tube 3 had the biggest contribution when tubes 0-3 were tested together, I thought it could be a kind of breaking point of the phototactic behavior.
As you can see in figure 1 there is a significant difference in the proportions between tube 5 and 3. The distribution of the flies in tube 5 are, like in the previous experiment, very similar to the experiment where all flies were tested, which is not the case for tube 3. Also in figure 2, where the variability is shown, it is possible to see a big difference between the distribution of flies of tube 5 and 3, which implies a difference in the behavior of those flies.
To complete the Experiment I tested the tubes 4, 2, 1 and 0 (Fig. 3 & 4)