Tdc2 and Bruchpilot in flight
on Tuesday, March 26th, 2013 7:00 | by Christine Damrau
Tested the third cross. Now, it is around 15n each group. After a control experiment I preferred a recovery time of 1h instead of 5h. A short pilot for control groups showed that Tdc2virgins crossed to w1118males have a flight defect.
3rd
Category: Biogenic Amines, flight
And your interpretation of the data so far is ? All experiments done in parallel, right? why did you test the w118 crosses ?
Of course, everything is tested in parallel (the data of the pilot control experiments are taken out).
We tested w1118 crosses to check for the effect of the eye color. The RNAi-Tdc2 progeny has lighter eyes. Because the w1118 crosses show a flight defect it seems like the lighter eyes correlate with less flight. In the next cross I will cross Tdc2 to another RNAi line, known to fly well, to proof that it is not the RNAi insertion alone giving the effect.
Interpretation SO FAR: bruchpilot does not seem to be necessary for octopamine signalling. Because the new results are not fitting to the previous ones I will wait for the next cross to give a final conclusion.
Everything I see in this graph looks like background effects now, if I’m, reading it correctly? That’s what you mean by ‘new results are not fitting to the previous ones’?
Yes. In the previous experiments, the Tdc-brpRNAi flies showed a flight defect (in both lines) whereas their controls flew fine. Now, the controls correlate with the experimental groups.
Let’s wait for the next cross to discuss the final conclusion.